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Behrouz Shiran Soghra Kiani Deepmala Sehgal Akram Hafizi Tanvir ul-Hassan Manju Chaudhary Soom Nath Raina 《Genetic Resources and Crop Evolution》2014,61(5):909-925
The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study. 相似文献
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Jyoti Kumar Bhupendra Nath Tripathi Rajiv Kumar Ganesh Gangaram Sonawane Shivendra Kumar Dixit 《Tropical animal health and production》2013,45(6):1429-1435
Corynebacterium pseudotuberculosis, a Gram-positive bacterium is the causative agent of caseous lymphadenitis (CLA), a chronic disease of sheep, goats and other warm blooded animals. In the present study, a total of 1,080 sheep reared under semi-intensive system on organized farms situated in the semi arid tropical region of Rajasthan, India, was clinically examined. Pus samples from superficial lymph nodes of 25 (2.31 %) adult sheep showing clinical lesions similar to CLA were collected for laboratory analyses. On the basis of morphological, cultural and biochemical characteristics 12 (48 %) bacterial isolates from pus identified it as C. pseudotuberculosis. A polymerase chain reaction (PCR) assay targeting Putative oligopeptide/dipeptide ABC transporter, nicotinamide adenine dinucleotide phosphate (NADP) oxidoreductase coenzyme F420-dependent and proline iminopeptidase (PIP) genes of C. pseudotuberculosis was developed that showed 14 pus samples as positive. All C. pseudotuberculosis isolates were also found positive for these genes in the PCR. The specificity of the PCR products was confirmed by sequencing of the amplified products that showed 98–100 % homology with published sequences available in the NCBI database. The present study shows the incidence of CLA as 2.31 %, 1.1 % and 1.29 % based on clinical, bacterial culture and direct pus PCR assay, respectively. The PCR assay was rapid, specific and as significant as bacterial culture in detecting bacteria directly in the clinical pus samples. The PCR assay developed in the study can be applied for the diagnosis and control of CLA. Furthermore, the assay can also be applied to detect C. pseudotuberculosis in various clinical samples. 相似文献
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B. G. Nath P. K. Pathak S. V. Ngachan A. K. Tripathi A. K. Mohanty 《Tropical animal health and production》2013,45(7):1513-1518
The present study was conducted to know the smallholder pig production system in tribal areas of Sikkim State, India. Two hundred tribal farmers were selected randomly from the North and East District of the state. Information on socio-economic characteristics of farmers (gender, occupation, educational status, and farming experience), management practices, disease prevalence, and economics in pig production was collected. The study recorded the mean land holding as 1.2?±?0.8 ha, and the number of pigs per farm was 5.0?±?0.28. Pigs were mainly kept as a source of income, and 70 % of farmers reared crossbreed pigs. Ninety percent (90 %) of respondents practiced the intensive system of management whereby kitchen wastes along with cooked mixture comprising maize bhusa, mustard oil cake, pseudostem of banana, tuber, stem, and plant leaves were used to feed their animals. About 40.5 % of farmers procured their breeding stock from government farms that had good records and utilized veterinary services like timely vaccination and deworming. The diseases prevalent in the study area were swine fever, diarrhea, helminthoses, sarcoptic mange, pneumonia, etc. The litter sizes at birth (local, 4.3?±?0.45; crossbreed, 7.2?±?0.33), at weaning (local, 2.79?±?0.24; crossbreed, 6.1?±?0.21), and age at first farrowing (local, 365.39?±?7.96 days; crossbreed, 337.24?±?8.79 days) were recorded. Production costs of meat extracted from local and crossbred pigs were 1.08 $/kg and 0.86 $/kg, respectively. 相似文献
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Paul M Bartley Frank Katzer Mara S Rocchi Stephen W Maley Julio Benavides Mintu Nath Yvonne Pang Germán Cantón Jackie Thomson Francesca Chianini Elisabeth A Innes 《Veterinary research》2013,44(1):91
This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses. 相似文献
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SummaryA field experiment was conducted on mango (Mangifera indica L.) to study the effects of farmyard manure (FYM) on soil organic carbon (SOC) content and on the build-up of fertility over three growing seasons (2006–2007, 2007–2008, and 2008–2009) at Bhubaneswar, Orissa, India. Five treatments, including the application of FYM at 5.0 kg plant–1, 7.5 kg plant–1, or 10.0 kg plant–1, the recommended dose of a chemical fertiliser (RDF; N:P:K=75:20:60), and a non-fertilised control, were evaluated in a randomised block design. The results indicated that SOC contents increased significantly in the FYM-treated plots. The highest SOC density (554 g m–2) and stock (5.55 Mg ha–1) were recorded in the treatment with FYM at 10 kg plant–1. The rate of increase in SOC density was highest (193 g m–2 year–1) with FYM at 10 kg plant–1, and lowest in the untreated control (15 g m–2 year–1). Regular addition of FYM had a positive effect on the build-up of soil fertility. However, the greatest increases in soil N, P, K contents were in the 7.5 kg plant–1 FYM treatment (means = 319.9, 19.1, and 270.8 kg ha–1 for N, P, K, respectively). Farmyard manure significantly influenced the growth parameters of mango trees over the three seasons. There was a positive and linear relationship between increasing rates of application of FYM and trunk cross-sectional area. 相似文献
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2008年2-4月,在印度西孟加拉邦德赖地区开展了试验,该试验对6个生长基质和3个种子的重量进行了分析,以找出其对梨竹萌发和实生苗生长的影响。与其他基质相比,将50 g的种子种在土壤、沙土和FYM以同一比例混合的基质中,种子萌发较好,实生苗生长更好。 相似文献